SG3ΔENV IN D116A

LOCUS       SG3ΔENV IN D116A               14788 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  Non-infectious HIV-1 Clone with stop codon in Env (see NIH AIDS 
            Reagent Program Cat #11051) and D116A mutation in integrase (IN 
            D116A)..
ACCESSION   .
VERSION     .
KEYWORDS    SG3-Delta-ENV IN D116A
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14788)
  AUTHORS   Christensen DE, Ganser-Pornillos BK, Johnson JS, Pornillos O, 
            Sundquist WI
  TITLE     Reconstitution and visualization of HIV-1 capsid-dependent 
            replication and integration in vitro.
  JOURNAL   Science. 2020 Oct 9;370(6513). pii: 370/6513/eabc8420. doi: 
            10.1126/science.abc8420.
  PUBMED    33033190
REFERENCE   2  (bases 1 to 14788)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 13, 2021 from SnapGene Server 1.1.58
            http://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..14788
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    3928..4045
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     RBS             5835..5846
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     misc_feature    6928..7161
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7346..7390
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     LTR             8622..8878
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     RBS             9079..9087
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     primer_bind     complement(9367..9386)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9368..9386)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9405..9421)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9405..9421)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9418..9440)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9429..9445
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(9453..9483)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9498..9519
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9636..9653)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9807..10395)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9887..9906)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(10566..11426)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11189..11208
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11427..11531)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      11558..12013
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11645..11664)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11855..11876
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     12139..12161
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     12153..12170
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     12154..12170
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     LTR             14313..14569
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    14616..14741
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
ORIGIN
        1 ggccaggggg aaagaaaaaa tataaattaa aacatctagt atgggcaagc agggagctag
       61 aacgattcgc agttaatccc ggcctgttag aaacagcaga aggctgtaga caaatactgg
      121 gacagctaca accgtccctt cagacaggat cagaagaact taaatcatta tataatacaa
      181 tagcagtcct ctattgtgtg catcaaatga tagatgtaaa agacaccaag gaagctttag
      241 agaagataga ggaagagcaa aacaacagta agaaaaaagc acagcaagca gcagctgaca
      301 caggaaacag cagccaggtc agccgaaatt accctatagt gcagaacatc caggggcaaa
      361 tggtacatca ggccatatca cccagaactt taaatgcatg ggtaaaagta gtagaagaga
      421 aggctttcag cccagaagta atacccatgt tttcagcatt atcagaagga gccaccccac
      481 aagatttaaa caccatgcta aacacagtgg ggggacatca agcagctatg caaatgttaa
      541 aagagaccat caatgaggaa gctgcagaat gggatagatt gcatccagtg caagcagggc
      601 ctgttgcacc aggccagatg agagaaccaa ggggaagtga catagcagga actactagta
      661 cccttcagga acaaatagga tggatgacaa ataatccacc tatcccagta ggagaaatct
      721 ataaaagatg gataatcctg ggattaaata aaatagtaag gatgtatagt ccttctagca
      781 ttctggacat aagacaagga ccaaaggaat cctttagaga ctatgtagac aggttctata
      841 aaactttaag agccgagcaa gcttcacagg aggtaaaaaa ttggatgaca gaaaccttgt
      901 tggtccaaaa tgcgaaccca gattgtaaga ccattttaaa agcattggga ccaggagcta
      961 cactagagga aatgatgaca gcatgtcagg gagtgggagg acccggccac aaagcaagag
     1021 ttttggcgga agcaatgagc caagtaacaa attcagccac cataatgatg cagagaggca
     1081 attttaggaa ccaaagaaag actgttaagt gtttcaattg tggcaaagaa gggcatatag
     1141 ccagaaattg caaggcccct aggaaaaggg gctgctggaa atgtggaaag gaaggacacc
     1201 aaatgaaaga ttgtacagag agacaggcta attttttagg gaagatctgg ccttcccaca
     1261 agggaaggcc agggaatttt cctcagagca gactagagcc aacagctcca ccagaagaga
     1321 gcttcaggtt tggggaggag acaacaactc cccctcagaa gcaggagcgg gaagacaagg
     1381 aaatgtatcc cttagcttcc ctcagatcac tctttggcaa cgacccctcg tcacaataaa
     1441 gatagggggg caactaaagg aggctctatt agatacagga gcagatgata cagtattaga
     1501 agaaatgaat ttgccaggga gatggaagcc aaaaatgata gggggaattg gaggttttat
     1561 caaagtaaga cagtatgatc agatacccat agaaatctgt ggacataaag ctataggtac
     1621 agtattagta ggacctacac ctgtcaacat aattggaaga aatctgttga ctcagattgg
     1681 ttgcactcta aattttccca ttagtcctat tgaaactgta ccagtaaaat taaagccagg
     1741 aatggatggc ccaaaagtta aacaatggcc attgacagaa gaaaaaataa aagcattagt
     1801 agaaatttgt acagaaatgg aaaaggaagg gaagatttca aaaattgggc ctgaaaatcc
     1861 atacaatact ccagtatttg ctataaagaa aaaagacagt actaaatgga gaaaattagt
     1921 agatttcaga gaacttaata agaggactca agacttctgg gaagttcaat taggaatacc
     1981 acatcccgct ggattaaaaa agaaaaaatc agtaacagta ctagatgtgg gtgatgcata
     2041 tttctcagtt cccttagata aagacttcag gaaatatact gcatttacca tacctagtat
     2101 aaacaatgag acaccaggga ttagatatca gtacaatgtg ctcccacagg gatggaaagg
     2161 atcaccagca atattccaaa gtagcatgac aaaaatctta gagcctttta gaaagcaaaa
     2221 tccagacata gttatctatc agtacatgga tgatttgtat gtaggatctg acttagaaat
     2281 agggcagcat agaacaaaaa tagaggaact gagacaacat ctgttaaggt ggggatttac
     2341 cacaccagac aaaaaacatc agaaagaacc tccattcctt tggatgggtt atgaactcca
     2401 tcctgataaa tggacagtac agcctatagt gctgccagaa aaagacagct ggactgtcaa
     2461 tgacatacag aagttagtgg gaaaattgaa ttgggcaagt cagatttact cagggatcaa
     2521 agtgaagcag ttatgtaaac tccttagggg aaccaaagca ctaacagaag tagtaacact
     2581 aacagaagaa gcagagctag aactggcaga aaacagggaa attctaaaag aaccagtaca
     2641 tggagtgtat tatgacccat caaaagactt aatagcagaa atacagaaac aggggcaagg
     2701 ccaatggaca tatcaaattt atcaagagcc atttaaaaat ctgaaaacag gaaaatatgc
     2761 aagaacgagg ggtgcccaca ctaatgatgt aaaacaatta acagaggcag tgcaaaaaat
     2821 aaccacagaa tgcataataa tatggggaaa aactcctaaa tttagactgc ccatacaaaa
     2881 agaaacatgg gaaacatggt ggacagagta ttggcaagcc acctggattc ctgaatggga
     2941 gtttgtcaat acccctccct tagtgaaatt atggtaccag ttagagaaag agcccataga
     3001 aggcgcagaa actttctatg tagatggagc agctaacagg gagactaaat taggaaaagc
     3061 aggatatgtt actaacaaag gaagacaaaa agttgtcacc ctaactgaca caacaaatca
     3121 gaagactgag ttagaagcaa ttcatctagc tttgcaggat tctggattag aagtaaacat
     3181 agtaacagac tcacaatatg cattaggaat cattcaagca caaccagata aaagtgaatc
     3241 agaattagtc agtcaaataa tagagcagtt aataaaaaag gaaaaggtct acctggcatg
     3301 ggtaccagca cacaaaggaa ttggaggaaa tgaacaagta gataaattag tcagtgctgg
     3361 ggtcaggaaa gtactatttt tagatggaat agataaggcc caagaagaac atgagaaata
     3421 tcacagtaat tggagagcca tggctagtga ttttaactta ccacctgtag tagcaaaaga
     3481 aatagtagcc agctgtgata aatgtcagct aaaaggagaa gccatgcatg gacaagtaga
     3541 ctgtagtcca ggaatatggc aactagattg cacacatcta gaaggaaaaa ttatcctggt
     3601 ggcggttcat gtagccagtg gatatataga agcagaagtt attccagcag agacagggca
     3661 ggaaacagca tactttctct taaaattagc aggaagatgg ccagtaaaaa caatacatac
     3721 agccaatggc agcaatttca ccagtaccac ggttaaggcc gcctgttggt gggcagggat
     3781 caagcaggaa tttggcattc cctacaatcc ccaaagtcaa ggagtagtag aatctatgaa
     3841 taaagaatta aagaaaatta taggacaggt aagagatcag gctgaacatc ttaaaacagc
     3901 agtacaaatg gcagtattta tccacaattt taaaagaaaa ggggggattg gggggtacag
     3961 tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag aactacaaaa
     4021 acaaattaca aaaattcaaa attttcgggt ttattacagg gacaacaaag atccactttg
     4081 gaaaggacca gcaaagcttc tctggaaagg tgaaggggca gtagtaatac aagataatag
     4141 tgacataaaa gtagtgccaa gaagaaaagc aaagatcatt agagattatg gaaaacagat
     4201 ggcaggtgat gattgtgtgg caagtagaca ggatgaggat tagaacatgg ataagtttag
     4261 taaaacacca tatgtatatt tcaaggaaag caaagggatg gttttataga catcactatg
     4321 aaagcactca cccaaaaata agttcagaag tacacatccc actaggggat gctagattgg
     4381 taataacaac atattggggt ctgcatacag gagaaagaga ttggcatttg ggtcatggag
     4441 tctccgtaga atggaggaaa aagagatata gcacacaagt agaccctgac ctagcagacc
     4501 aactaattca tctgtattac tttgattgtt tttcagaatc tgccataaga aatgccatat
     4561 taggacatat agttagtcct aggtgtgaat atcaagcagg acataacaag gtaggatctc
     4621 tacagtacct agcactagca gcattaataa caccaaaaag gataaagcca cctttgccta
     4681 gtgttacaaa actaacagag gatagatgga acaagcccca gaagaccaag ggccacagag
     4741 ggagccatac aatgaatgga cactagagct tttagaagaa cttaagaatg aagctgttag
     4801 acattttcct aggatatggc tccatggctt agggcaatat atctatgaaa cttatgggga
     4861 tacttgggca ggagtggaag ccctagtaag aactctgcaa caactgctgt ttactctttt
     4921 cagaattggg tgtcgacata gcagaatagg cattactcaa cgaagaagag caagaaatgg
     4981 agccagtaga tcctagacta gagccctgga agcatccagg aagccagcct aaaactgctt
     5041 gtaccaaatg ctattgtaaa aagtgttgct tacattgcca agtttgtttc atgacaaaag
     5101 gcttaggcat ctcctatggc aggaagaagc ggagacagcg acgaagagct cctcaagaca
     5161 gtcaggctca tcaagcttct ctatcaaagc agtaagtagt gcatgtaatg caacctatac
     5221 aaatagcagc aatagtagca ttagtagtgg taggaataat agcaatagtt gtgtggtcca
     5281 tagtattaat agaatatagg aaaatattaa gacaaagaaa aatagacagg ttaattaaaa
     5341 gaataagtaa aagagcagaa gacagtggca atgagagtga aggagatcag gaagaattat
     5401 cagcacttgt ggagatgggg caccatgctc tttgggatat tgatgatcta tagtgctgca
     5461 gggaatttgt gggtcacagt atattatggg gtacctgtat ggaaagaagc aaccaccact
     5521 ctattttgtg catcagatgc taaagcatat gatacagagg tacataatgt ttgggccaca
     5581 catgcctgta tacccacaga ccccaaccca caagagatag tattagaaaa tgtgacagaa
     5641 gattttaaca tgtggaaaaa taacatggta gaacagatgc aggaggatat aatcagttta
     5701 tgggatcaaa gcctaaagcc atgtgtaaaa ttaaccccac tctgtgttac tctaaattgc
     5761 actgatgagt tgaagaatgc tactaatacc actagctagt actaataccc ctagtggtag
     5821 ctggaaaaag atggaaagag gagaaataaa gaactgctct ttcaatgtct taggagataa
     5881 gaagcagaaa gcatatgcac tcttttataa acttgatgta gtaccaatag ataatgataa
     5941 gaatagtacc aaatataggt tgataagttg taacacctca gtcactacac aggcctgtcc
     6001 aaagatatcc tttgagccaa ttcccataca ttattgtgcc ccggctggtt ttgcgattct
     6061 aaagtgtaaa gataagaagt tcaatggaac aggatcatgt aaaaatgtca gcacagtaca
     6121 atgtacacat ggaattaagc cagtagtatc aactcaactg ctgttaaatg gcagcctagc
     6181 agaagaagag gtagtaatta gatctgccaa tttctcggac aatgctaaaa ccataatagt
     6241 acagctgaaa gaagctgtag aaattaattg cacaagaccc aacaaaaaaa caagaaaacg
     6301 tataactacg ggaccaggga gagtatatta tacaacagga gaaatagtag gagatataag
     6361 acaagcacat tgtaacctta gtagagcaaa atggaatgac actttaagac agatagttat
     6421 aaaattaaga gaacaatttg agaataaaac aatagtcttt aatcaatcct caggagggga
     6481 cccagaaatt gtaatgcata gttttaattg tggaggggaa tttttctact gtaagtcaac
     6541 acaactgttt aatagtactt gggctgggaa taatacttgg aatagtagtg ctgaaaggtc
     6601 agatgacact ggaggaaata tcacactccc atgcagaata aaacaaatta taaacatgtg
     6661 gcaggaagta ggaaaagcaa tgtatgcccc tcccatcagc ggacaaatta gatgtacatc
     6721 aaatattaca gggctgctat taacaagaga tggtggtacg agcgatacca ataccaccga
     6781 gaccttcaga cctggaggag gagatatgag ggacaattgg agaagtgaat tatataaata
     6841 taaagtagta aaaattgaac cattaggagt agcacccacc acggcaaaga gaagagtggt
     6901 gcaaagagaa aaaagagcag tgggaatagg agctctgttc cttgggttct tgggagcagc
     6961 aggaagcact atgggcgcag cgtcaatgac gttgacggta caggccagac aattattgtc
     7021 tggtatagtg caacagcaga acaatttgct gagggctatt gaggcgcaac agcatctgtt
     7081 gcaactcaca gtctggggca tcaagcagct ccaggcaaga gtcctggctg tggaaagata
     7141 cctaaaggat caacagctcc tggggatttg gggttgctct ggaaaactca tttgcaccac
     7201 tgctgtgcct tggaatgcta gttggagtaa taaatctctg aatcagattt gggataacat
     7261 gacttggatg cagtgggaaa gagaaattga aaattacaca gacttaatat acaacttaat
     7321 tgaagaatcg cagaaccagc aagaaaagaa tgaacaagaa ttattggaat tagataaatg
     7381 ggcaagtttg tggaattggt ttacaataac aaactggctg tggtatataa aaatattcat
     7441 aatgatagta ggaggcttga taggtttaag aatagttttt actgtacttt ctatagtgaa
     7501 tagagttagg cagggatact caccattgtc gtttcagacc cacctcccaa ccccgagggg
     7561 acccgacagg cccgaaggaa tcgaagaaga aggtggagag agagacagag acagatccgg
     7621 tcgattagtg aacggattct tagcactttt ctgggacgat ctgcggagcc tgtgcctctt
     7681 cagctaccac cgcttgagag acttaatctt ggttgtaacg aggattgtgg aacttctggg
     7741 acgcaggggg tgggaagccc tcaagtattg gtggagtctc ctacagtatt ggagccagga
     7801 actaaagaat agtgctgtta acttgcttaa tgtcacagcc atagcagtag ctgagggaac
     7861 agatagggtt atagaagtag tacaaagaac ttatagagct attctccaca tacctagaag
     7921 aataagacag ggcttggaaa ggcttttgct ataagatggg tggcaagtgg tcaaaacgta
     7981 tggagggtgg atggcatgct gtaagggaaa gaatgagacg agctgagcca gcagcagatg
     8041 gggtgggagc agtatctaga gacctggaaa aacatggagc aatcacaagt agcaatacag
     8101 cagctaacaa tgctgattgt gcctggctgg aagcacaaga ggaggaggag gtgggttttc
     8161 cagtcagacc tcaggtacct ttaagaccaa taacttacaa ggcagctgta gatattagcc
     8221 actttttaaa agaaaagggg ggactggaag ggctaatttt ctcccaaaga agacaagata
     8281 tccttgatct gtggacctac cacacacaag gctacttccc tgattggcag aactacacac
     8341 cagggccagg gatcagatat ccactgacct ttggatggtg tttcaagcta gtaccagttg
     8401 atccagagaa gatagaagag gccaatgaag gagaaaacaa cagcttgcta caccctatga
     8461 gccagcatgg gatggatgac ccggagagag aagtgctaga gtggaggttt gacagccgcc
     8521 tagcatttca tcacatggcc cgagagctgc atccggagta ctacaagaac cgatgacatc
     8581 gcgctttcta caagggactt tccgctgggg actttccagg ggaggcgtgg cctgggcggg
     8641 accggggagt ggcgagccct cagatgctgc atataagcag ctgcttttgc ctgtactggg
     8701 tctctctggt tagaccagat ctgagcctgg gagctctctg gctagctagg gaacccactg
     8761 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     8821 gactctggta gctagagatc cctcataccc ttttagtcag tgtggaaaat ctctagcaaa
     8881 aattcctagc tgcctagaaa taaaacacta tgcttttgaa taacatatga cttaaaagaa
     8941 gaatttatga tggctattag aaaatatttt aaactgattg aaaatgaagg tgaactacaa
     9001 tgatctagat tgctgctgct gaaatgctaa gatgaaattt atagccttaa gtctatgcat
     9061 tggaaaagaa agctttttta aggaggtaga aaaggaatag gaaatcaaac ctaaagacag
     9121 tagaagtaaa gaaataatga agacaagagc agaaattagt gaatagaaag taatcttaca
     9181 tagagaaaat tgacaaagct aagaaaaagt tggttctttg aaaagattaa tgtaattgac
     9241 cacttagcaa aactgataaa aggaggatgg ggagggggag agagaacata aatttaaata
     9301 ataggaattg aaaaatgtca catcactacg gatcctctag agtcgacctg caggcatgca
     9361 agctttccct atagtgagtc gtattagagc ttggcgtaat catggtcata gctgtttcct
     9421 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     9481 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     9541 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     9601 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     9661 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     9721 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     9781 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     9841 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     9901 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     9961 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
    10021 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
    10081 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
    10141 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
    10201 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
    10261 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
    10321 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
    10381 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
    10441 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
    10501 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
    10561 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
    10621 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
    10681 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
    10741 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
    10801 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
    10861 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
    10921 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
    10981 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
    11041 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
    11101 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
    11161 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
    11221 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
    11281 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
    11341 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
    11401 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
    11461 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
    11521 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg cgccctgtag cggcgcatta
    11581 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
    11641 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
    11701 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
    11761 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
    11821 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
    11881 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
    11941 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta
    12001 acgcttacaa tttccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc
    12061 gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt
    12121 gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt gaattccttc
    12181 tatggaatac tttttaaata tatagtctga taatatataa ctgacacgtt ttctgttgtt
    12241 tcataatggt tgattttctc agggctacgt ttctctattt tttcctgttc tcaaatctta
    12301 actctccatc tctgtcctca agtggtaagt gatcttgtgt cttattttga aattaaagtc
    12361 atcaacaaat tatttcaact catagattta tagttgtgga agacttacta ttcaaatgga
    12421 attagaacgt tttgggggat aggatgcagt agttcttggt agtccaagac tcttaataaa
    12481 acaactagaa aagccaggta atttacaaaa tttatatatt tagcgataga gaacaatggg
    12541 tgtaaaaatg attaaagaaa gcaaaatttt gcaccctggg ggaatacctt tcacaagtga
    12601 gctgagaggt ctgcagctga tcattgtttt tcctgggtac agagtagagg aggcctttgg
    12661 gctgcagcct gagtggcaga ttggaaatat gaggggcctt taaacacaga gaacgccctc
    12721 agtatattcc taaattctgt agttcagctg ggcggaagag ataaaccaaa atctcagaaa
    12781 aggagattgg gatattttct agtcctatgg tgttgagata aagattctct aggggaagcg
    12841 acttgagaac aataggtgag agttgaaagc gtttgaggaa gtggtgaata cacactgatg
    12901 attttcaaca cacccagttt cttcccccgt agacatttgc caaattctga agctgcttag
    12961 gctagagagc taaaccagaa gtctcttaaa aacaaagcaa ttcttatagt atctaggtac
    13021 ctagaagata acgatctgct agcttttgaa atgaaggctc tgcagagtct catcctagga
    13081 acagggacaa accagaggta gtctccttca tagcaaaact gctactcagc agtgttctat
    13141 gattctggca ttatagagga agtagtaaaa gtatatgtat gaaaattgtg ttaagtgaag
    13201 gatgaataag aaaatatcta gggtaaccac taaaagaaaa gaatgtgtaa caaatgaata
    13261 cagaaaaaaa ttggatatag aatatgtgat aaattcagaa gaagacaaga aagaaaatat
    13321 aaaacaagca ggcaactaaa ttagtaatta tgttacatgg aaatgggcta aatatttcaa
    13381 gtaaatggca aaaatcatca gactggacaa ataataatta cctaaatata agtacctaga
    13441 aagattgaga gaaaatgatg cgaaaaggta taccatccaa atgctaactg aaggaaataa
    13501 gtgtggctat accaatcagg caaaggagac tttaatacaa taaacagtag tagaaaaaga
    13561 gaactacatt ttatagtgta aggggattga tccaccagag ttaccacaat cctttatgtg
    13621 tacccagtaa aatagcccaa acatctataa accaaaaatt ttattttgaa ctaaaagaaa
    13681 cagacaaata tgccctcaca atggatgcac atatatagga catacataca tcacaattag
    13741 caggcttgat ctaattgtca tacgtagaac attgaaacta ttaatggtgg tattaaccat
    13801 cttttccctt gcacatggaa attgtactag aattgaccat aagctagggc catgaacaaa
    13861 gcctcagcaa atatcattga ttttaataat tgaatatgtt gtttgtatta tagtaaataa
    13921 aaataactag aaaattggaa gggctaatta tctcccaaag aagacaagat atccttgatc
    13981 tgtggaccta ccacacacaa ggctacttcc ctgattggca gaactacaca ccagggccag
    14041 ggatcagata tccactgacc tttggatggt gtttcaagct agtaccagtt gatccagaga
    14101 agatagaaga ggccaatgaa ggagaaaaca acagcttgct acaccctatg agccagcatg
    14161 ggatggatga cccggagaga gaagtgctag agtggaggtt tgacagccgc ctagcatttc
    14221 atcacatggc ccgagagctg catccggagt actacaagaa ccgatgacat cgcgctttct
    14281 acaagggact ttccgctggg gactttccag gggaggcgtg gcctgggcgg gaccggggag
    14341 tggcgagccc tcagatgctg catataagca gctgcttttg cctgtactgg gtctctctgg
    14401 ttagaccaga tctgagcctg ggagctctct ggctagctag ggaacccact gcttaagcct
    14461 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
    14521 agctagagat ccctcatacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga
    14581 acagggaccg gaaagcgaaa gagaaaccag aggagctctc tcgacgcagg actcggcttg
    14641 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
    14701 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattgag cgggggaaaa
    14761 ttggataagt gggagaaaat tcggttaa
//